T Lymphocyte Subsets and Cytokines in Rats Transplanted with Adipose-Derived Mesenchymal Stem Cells and Acellular Nerve for Repairing the Nerve Defects
Journal of Korean Neurosurgical Society
;
: 101-106, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-211053
ABSTRACT
OBJECTIVE:
The aim of this study was to explore the immunity in rats transplanted with adipose-derived mesenchymal stem cells (ADSCs) and acellular nerve (ACN) for repairing sciatic nerve defects.METHODS:
ADSCs were isolated from the adipose tissues of Wistar rats. Sprague-Dawley rats were used to establish a sciatic nerve defect model and then divided into four groups, according to the followingmethods:
Group A, allogenic nerve graft; Group B, allograft with ACN; Group C, allograft ADSCs+ACN, and Group D, nerve autograft.RESULTS:
At the day before transplantation and 3, 7, 14, and 28 days after transplantation, orbital venous blood of the Sprague-Dawley rats in each group was collected to detect the proportion of CD3+, CD4+, and CD8+ subsets using flow cytometry and to determine the serum concentration of interleukin-2 (IL-2), tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) using enzyme-linked immunosorbent assay (ELISA). At each postoperative time point, the proportion of CD3+, CD4+, and CD8+ subsets and the serum concentration of IL-2, TNF-alpha, and IFN-gamma in group C were all near to those in group B and group D, in which no statistically significant difference was observed. As compared with group A, the proportion of CD3+, CD4+, and CD8+ subsets and the serum concentration of IL-2, TNF-alpha, and IFN-gamma were significantly reduced in group C (p<0.05).CONCLUSION:
The artificial nerve established with ADSCs and ACN has no obvious allograft rejection for repairing rat nerve defects.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Órbita
/
Nervio Ciático
/
Ensayo de Inmunoadsorción Enzimática
/
Citocinas
/
Subgrupos de Linfocitos T
/
Interferón gamma
/
Interleucina-2
/
Factor de Necrosis Tumoral alfa
/
Ratas Wistar
/
Ratas Sprague-Dawley
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Journal of Korean Neurosurgical Society
Año:
2015
Tipo del documento:
Artículo
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