Conservation of cis-Regulatory Element Controlling Timely Translation in the 3'-UTR of Selected Mammalian Maternal Transcripts
Genomics & Informatics
; : 174-178, 2007.
Article
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| WPRIM
| ID: wpr-21117
Biblioteca responsable:
WPRO
ABSTRACT
The earliest stages of mammalian embryogenesis are governed by the activity of maternally inherited transcripts and proteins. Cytoplasmic polyadenylation of selected maternal mRNA has been reported to be a major control mechanism of delayed translation during preimplantation embryogenesis in mice. The presence of cis-elements required for cytoplasmic polyadenylation (e.g., CPE) can serve as a useful tag in the screening of maternal genes partaking in key functions in the transcriptionally dormant egg and early embryo. However, due to its relative simplicity, UA-rich sequences satisfying the canonical rule of known CPE consensus sequences are often found in the 3'-UTR of maternal transcripts that do not actually undergo cytoplasmic polyadenylation. In this study, we developed a method to confirm the validity of candidate CPE sequences in a given gene by a multiplex comparison of 3'-UTR sequences between mammalian homologs. We found that genes undergoing cytoplasmic polyadenylation tend to create a conserved block around the CPE, while CPE-like sequences in the 3'-UTR of genes lacking cytoplasmic polyadenylation do not exhibit such conservation between species. Through this cross-species comparison, we also identified an alternative CPE in the 3'-UTR of tissue-type plasminogen activator (tPA), which is more likely to serve as a functional element. We suggest that verification of CPEs based on sequence conservation can provide a convenient tool for mass screening of factors governing the earliest processes of mammalian embryogenesis.
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Texto completo:
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Índice:
WPRIM
Asunto principal:
Óvulo
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Tamizaje Masivo
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Secuencia de Consenso
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Activador de Tejido Plasminógeno
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ARN Mensajero Almacenado
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Citoplasma
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Poliadenilación
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Desarrollo Embrionario
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Estructuras Embrionarias
Tipo de estudio:
Prognostic_studies
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Screening_studies
Límite:
Animals
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Pregnancy
Idioma:
En
Revista:
Genomics & Informatics
Año:
2007
Tipo del documento:
Article