Conservation of cis-Regulatory Element Controlling Timely Translation in the 3'-UTR of Selected Mammalian Maternal Transcripts
Genomics & Informatics
;
: 174-178, 2007.
Artículo
en Inglés
| WPRIM
| ID: wpr-21117
ABSTRACT
The earliest stages of mammalian embryogenesis are governed by the activity of maternally inherited transcripts and proteins. Cytoplasmic polyadenylation of selected maternal mRNA has been reported to be a major control mechanism of delayed translation during preimplantation embryogenesis in mice. The presence of cis-elements required for cytoplasmic polyadenylation (e.g., CPE) can serve as a useful tag in the screening of maternal genes partaking in key functions in the transcriptionally dormant egg and early embryo. However, due to its relative simplicity, UA-rich sequences satisfying the canonical rule of known CPE consensus sequences are often found in the 3'-UTR of maternal transcripts that do not actually undergo cytoplasmic polyadenylation. In this study, we developed a method to confirm the validity of candidate CPE sequences in a given gene by a multiplex comparison of 3'-UTR sequences between mammalian homologs. We found that genes undergoing cytoplasmic polyadenylation tend to create a conserved block around the CPE, while CPE-like sequences in the 3'-UTR of genes lacking cytoplasmic polyadenylation do not exhibit such conservation between species. Through this cross-species comparison, we also identified an alternative CPE in the 3'-UTR of tissue-type plasminogen activator (tPA), which is more likely to serve as a functional element. We suggest that verification of CPEs based on sequence conservation can provide a convenient tool for mass screening of factors governing the earliest processes of mammalian embryogenesis.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Óvulo
/
Tamizaje Masivo
/
Secuencia de Consenso
/
Activador de Tejido Plasminógeno
/
ARN Mensajero Almacenado
/
Citoplasma
/
Poliadenilación
/
Desarrollo Embrionario
/
Estructuras Embrionarias
Tipo de estudio:
Estudio pronóstico
/
Estudio de tamizaje
Límite:
Animales
/
Embarazo
Idioma:
Inglés
Revista:
Genomics & Informatics
Año:
2007
Tipo del documento:
Artículo
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