Differentiation of human labia minora dermis-derived fibroblasts into insulin-producing cells
Experimental & Molecular Medicine
;
: 26-35, 2012.
Artículo
en Inglés
| WPRIM
| ID: wpr-211721
ABSTRACT
Recent evidence has suggested that human skin fibroblasts may represent a novel source of therapeutic stem cells. In this study, we report a 3-stage method to induce the differentiation of skin fibroblasts into insulin-producing cells (IPCs). In stage 1, we establish the isolation, expansion and characterization of mesenchymal stem cells from human labia minora dermis-derived fibroblasts (hLMDFs) (stage 1 MSC expansion). hLMDFs express the typical mesenchymal stem cell marker proteins and can differentiate into adipocytes, osteoblasts, chondrocytes or muscle cells. In stage 2, DMEM/F12 serum-free medium with ITS mix (insulin, transferrin, and selenite) is used to induce differentiation of hLMDFs into endoderm-like cells, as determined by the expression of the endoderm markers Sox17, Foxa2, and PDX1 (stage 2 mesenchymal-endoderm transition). In stage 3, cells in the mesenchymal-endoderm transition stage are treated with nicotinamide in order to further differentiate into self-assembled, 3-dimensional islet cell-like clusters that express multiple genes related to pancreatic beta-cell development and function (stage 3 IPC). We also found that the transplantation of IPCs can normalize blood glucose levels and rescue glucose homeostasis in streptozotocin-induced diabetic mice. These results indicate that hLMDFs have the capacity to differentiate into functionally competent IPCs and represent a potential cell-based treatment for diabetes mellitus.
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Transferrina
/
Biomarcadores
/
Diferenciación Celular
/
Transactivadores
/
Separación Celular
/
Células Cultivadas
/
Trasplante de Islotes Pancreáticos
/
Niacinamida
/
Selenito de Sodio
/
Proteínas de Homeodominio
Límite:
Animales
/
Femenino
/
Humanos
Idioma:
Inglés
Revista:
Experimental & Molecular Medicine
Año:
2012
Tipo del documento:
Artículo
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