Human SNF2L Gene Is Regulated Constitutively and Inducibly in Neural Cells via a cAMP-Response Element
Yonsei Medical Journal
;
: 772-777, 2013.
Artículo
en Inglés
| WPRIM
| ID: wpr-211908
ABSTRACT
PURPOSE:
SNF2L belongs to Imitation Switch family and plays an essential role in neural tissues and gonads. In our previous studies, we have demonstrated that the basal transcription of human SNF2L gene is regulated by two cis-elements, cAMP response element (CRE)- and Sp1-binding sites. Recent studies suggested that cyclic adenosine monophosphate (cAMP) stimulation significantly up-regulated SNF2L expression in ovarian granulose cells. These data suggested that protein kinase-mediated signal pathways might also regulate SNF2L expression in neural cells. We therefore investigated the effects of agents that activate protein kinases A on SNF2L gene expression in neural cells. MATERIALS ANDMETHODS:
To increase intracellular cAMP levels, all neural cells were treated with forskolin and dbcAMP, two cAMP response activators. We exmined the effects of cAMP on the promoter activity of human SNF2L gene by luciferase reporter gene assays, and further examined the effects of cAMP on endogenous SNF2L mRNA levels by qPCR.RESULTS:
Transient expression of a luciferase fusion gene under the control of the SNF2L promoter was significantly increased by treatment of rat primary neurons with forskolin or dbcAMP, but not PC12, C6 and SH-SY5Y cells. Consistently, treatment with forskolin or dbcAMP could enhance endogenous SNF2L mRNA levels also only in rat primary neurons.CONCLUSION:
These results suggest that the CRE consensus sequence in the SNF2L proximal promoter most likely confers constitutive activation and regulation by cAMP in neural cells.
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Factores de Transcripción
/
Proteínas Recombinantes de Fusión
/
Colforsina
/
ARN Mensajero
/
Línea Celular
/
Regulación de la Expresión Génica
/
Regiones Promotoras Genéticas
/
Células PC12
/
Ratas Wistar
/
AMP Cíclico
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Yonsei Medical Journal
Año:
2013
Tipo del documento:
Artículo
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