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Inhibition of Neointima Formation by Anti-Vascular Endothelial Growth Factor and Receptor-1 Peptides in a Balloon-Injured Rat Carotid Artery
Korean Circulation Journal ; : 475-482, 2007.
Artículo en Inglés | WPRIM | ID: wpr-212720
ABSTRACT
BACKGROUND AND

OBJECTIVES:

Vascular endothelial growth factor (VEGF) is a potent endothelial cell-specific mitogen. This study was undertaken to test the hypothesis that the neointima hyperplasia induced by a balloon injury is inhibited by blocking VEGF and VEGF receptor-1 (VEGFR-1) with anti-VEGF peptides. Materials and

Methods:

Anti-VEGF RRKRRR peptide (dRK6) and anti-VEGFR-1 peptide (anti-flt-1) were synthesized at Pohang University of Science and Technology, Korea. Male Sprague-Dawley rats, weighing 300-350 g, were subcutaneously injected 0.5 mg/kg of dRK6 or 0.5 mg/kg of anti-flt-1, dissolved in phosphate buffer solution, 2 days before induction of a carotid balloon-injury, and then daily in the same manner post carotid balloon injury for 2 weeks.

RESULTS:

Neointima formation was suppressed in both the dRK6 and anti-flt-1 groups compared to that in the untreated controls at 2 weeks post carotid balloon-injury (neointimal area; control group 0.44+/-0.09 mm2, dRK6 group 0.25+/-0.05 mm2, anti-flt-1 group 0.19+/-0.05 mm2, p<0.01). Anti-flt-1 peptide and dRK6 reduced the numbers of proliferative bromodeoxyuridine-labeled cells in the neointima (control group 16.4+/-10.6%, dRK6 group 3.7+/-2.1%, anti-flt-1 group 5.9+/-3.4%, p<0.05). In addition, an inflammatory response, as determined by monocyte chemoattractant protein-1 and interleukin-6 upregulation, which was evident in the controls, was inhibited by both dRK6 and anti-flt-1.

CONCLUSION:

This study suggests anti-vascular endothelial growth factor peptides can reduce the inflammation and neointima formation in balloon injured rat carotid arteries.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Péptidos / Arterias Carótidas / Regulación hacia Arriba / Factores de Crecimiento Endotelial / Interleucina-6 / Ratas Sprague-Dawley / Quimiocina CCL2 / Traumatismos de las Arterias Carótidas / Receptor 1 de Factores de Crecimiento Endotelial Vascular / Factores de Crecimiento Endotelial Vascular Límite: Animales / Humanos / Masculino País/Región como asunto: Asia Idioma: Inglés Revista: Korean Circulation Journal Año: 2007 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Péptidos / Arterias Carótidas / Regulación hacia Arriba / Factores de Crecimiento Endotelial / Interleucina-6 / Ratas Sprague-Dawley / Quimiocina CCL2 / Traumatismos de las Arterias Carótidas / Receptor 1 de Factores de Crecimiento Endotelial Vascular / Factores de Crecimiento Endotelial Vascular Límite: Animales / Humanos / Masculino País/Región como asunto: Asia Idioma: Inglés Revista: Korean Circulation Journal Año: 2007 Tipo del documento: Artículo