A PCR-RFLP method for the detection of activated H-ras oncogene with a point mutation at codon 12 and 61
Yonsei Medical Journal
;
: 371-379, 1996.
Artículo
en Inglés
| WPRIM
| ID: wpr-213092
ABSTRACT
To investigate the incidence of the H-ras gene activation in bladder tumor and the feasibility of using urinary washout samples for screening, a series of 33 human bladder tumors and their preoperatively collected urinary washout samples were screened using a mutant specific PCR-RFLP (polymerase chain-restriction fragment length polymorphism) to detect a point mutation of the H-ras gene. Five tumors were found to harbor H-ras mutations where two tumors had a glycine to valine (G-->T) change in codon 12 and three tumors had a glutamine to lysine (C-->A) change in codon 61, respectively. Moreover, we could also detect the same point mutations of the H-ras gene in corresponding urine washout samples. The incidence of H-ras mutation in Korean bladder cancer was estimated at approximately 15.2%. In conclusion, a mutant specific PCR-RFLP method for the detection of H-ras gene mutation is useful for screening or postoperative follow-up of bladder tumor due to its simplicity and high specificity even in urinary samples.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Neoplasias de la Vejiga Urinaria
/
Polimorfismo de Longitud del Fragmento de Restricción
/
Codón
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Carcinoma de Células Transicionales
/
Regulación de la Expresión Génica
/
Reacción en Cadena de la Polimerasa
/
Genes ras
/
Mutación Puntual
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
Idioma:
Inglés
Revista:
Yonsei Medical Journal
Año:
1996
Tipo del documento:
Artículo
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