Role of the DNA Binding Domain of C/EBP epsilon on Granulopoiesis / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 205-211, 2005.
Artículo
en Coreano
| WPRIM
| ID: wpr-214441
ABSTRACT
BACKGROUND:
The CCAAT/enhancer binding protein epsilon (C/EBPepsilon), one of the transcription factors, plays an important role in granulopoiesis. We examined an essential site of C/EBP epsilon for granulopoiesis.METHODS:
32Dcl3 cells and #1111 cells were transduced with retroviral constructs of C/EBP epsilon and C/EBP epsilon(R211A) (DNA binding mutant form). We examined growth rate, checked the neutrophil markers of Gr-1 and Mac-1, and counted differentiated cells in the transduced 32Dcl3 cells. The transduced #1111 cells were injected into five mice and survival times were analyzed.RESULTS:
The mean number of green fluorescent protein (GFP) (+) 32Dcl3 cells transduced with C/EBP was 244, 045 at day 2, 582, 938 at day 4, and 873, 963 at day 6; mean expression of Gr-1 was 31.3% and Mac-1 32.6%; mean count of immature form was 41.0%, intermediate form 48.3%, and mature form 10.7%. In case of C/EBP epsilon (R211A) transduced 32Dcl3 cells, the respective figures were 707, 226, 1, 106, 736, and 2, 133, 819; 0.1% and 0.1%; and 91.7%, 4.3%, and 4.0%. The mean survival time of #1111 cells transduced with C/EBP epsilon was 26.0 days in placebo group and 34.0 days in 4-hydroxytamoxifen (4HT; C/EBP epsilon group); in case of C/EBP epsilon(R211A) transduced #1111 cells, the respective figures were 36.4 and 37.4 days.CONCLUSIONS:
The growth of 32Dcl3 and #1111 cells was inhibited by C/EBP epsilon, but not by C/EBP epsilon(R211A). Also C/EBP epsilon was involved in the differentiation of 32Dcl3 cells, but C/EBP epsilon(R211A) was not. The DNA binding domain of C/EBP epsilon is a very important site for differentiating and inhibiting early myeloid cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Factores de Transcripción
/
ADN
/
Zidovudina
/
Proteínas Portadoras
/
Tasa de Supervivencia
/
Células Mieloides
/
Neutrófilos
Límite:
Animales
Idioma:
Coreano
Revista:
The Korean Journal of Laboratory Medicine
Año:
2005
Tipo del documento:
Artículo
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