Genetic Variation in Exon 3 of Human Apo B mRNA Editing Protein (apobec-1) Gene
Journal of Genetic Medicine
;
: 15-20, 1999.
Artículo
en Inglés
| WPRIM
| ID: wpr-214517
ABSTRACT
We have investigated the genetic variation in the human apo B mRNA editing protein (apobec-1) gene. Exon 3 of the apobec-1 gene was amplified by polymerase chain reaction. After detection of an additional band by single strand conformational polymorphism (SSCP) analysis, sequencing of the SSCP shift sample revealed a single-base mutation. The mutation was a CGG transversion at codon 80 resulting in a lleRMet substitution. Thes substitution was confirmed by restriction fragment length polymorphism analysis since a pvull site is abolished by the substitution. Population and family studies confirmed that the inheritance of the genotypes for apobec-1 gene polyomrphism is comtrolled by two codominant alleles (P1 and P2). A significant difference in plasma triglyceride was detected among the different apobec-1 genotypes in the CAD patients (P<0.05) Our study could provide the basis for elucidating the interaction between gentic variation of the apobec-1 gene and disorders related to lipid metabolism.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Apolipoproteínas B
/
Plasma
/
Triglicéridos
/
Variación Genética
/
Testamentos
/
Polimorfismo de Longitud del Fragmento de Restricción
/
Codón
/
ARN Mensajero
/
Reacción en Cadena de la Polimerasa
/
Exones
Límite:
Humanos
Idioma:
Inglés
Revista:
Journal of Genetic Medicine
Año:
1999
Tipo del documento:
Artículo
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