Effects of Mycobacterium leprae , M . leprae Phenolic Glycolipid - 1 and Cytokines on the Nitric Oxide Generation of RAW 264 . 7 Macrophages / 대한피부과학회지
Korean Journal of Dermatology
;
: 234-244, 1994.
Artículo
en Coreano
| WPRIM
| ID: wpr-215134
ABSTRACT
Background:
Nitric oxide(NO) has been reproted to play an important role in macrophage-mediated microbicidal capacity for a variety of intracellular pathogens. NO generation is used as an indicator of microbicidal function of macrophages.OBJECTIVE:
Our purpose is to investigate the production of NO rom macrophages phagocytized with Mycobacterium leprae or M. leprae phenolic glycolipid-1(PGL-1) for the purpose of elucidating the pathogenesis of leprosy.METHODS:
We used a murine macrophage cell line, RAW 264.7. Macrophages were incubated with dead M. leprae or PGL-1, respectively and then treated with interfer n-gamma(IFN-r) and/or tumor necrosis factor-alpha(TNF-a). The release of NO was determined spectrophotometrically by measuring nitrite.RESULTS:
M. Leprae and PGL-1 failed to stimulnte NO secretion execept at high bacteria-to-cell rations(501)and at the higheat concentrat,ion(100pg/ml) of PGL-1. IFN-r or IFN-r plus TNF-a markedly stimulated macrophages phagocyt,ized with M. leprae or PGL-1 to release NO .CONCLUSION:
Defective IFN-r-dependent NO production of macrophages may be an important factor in the pathogenesis of leprosy.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Línea Celular
/
Citocinas
/
Fenol
/
Lepra
/
Macrófagos
/
Mycobacterium
/
Mycobacterium leprae
/
Necrosis
/
Óxido Nítrico
Idioma:
Coreano
Revista:
Korean Journal of Dermatology
Año:
1994
Tipo del documento:
Artículo
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