Your browser doesn't support javascript.
loading
The Effect of ATP-sensitive Potassium Channel on R-PIA Induced Mechanical Antiallodynia in a Peripheral Neuropathic Rat / 대한통증학회지
The Korean Journal of Pain ; : 107-112, 2005.
Artículo en Coreano | WPRIM | ID: wpr-215231
ABSTRACT

BACKGROUND:

Nerve ligation injury may produce mechanical allodynia, but this can be reversed after an intrathecal administration of adenosine analogues. In many animal and human studies, ATP-sensitive potassium channel blockers have been known to reverse the antinociceptive effect of various drugs. This study was performed to evaluate the mechanical antiallodynic effects of spinal R-PIA (Adenosine A1 receptor agonist) and the reversal of these effects due to pretreatment with glibenclamide (ATP-sensitive potassium channel blocker). Thus, the relationship between the antiallodynic effects of R-PIA and ATP-sensitive potassium channel were investigated in a neuropathic model.

METHODS:

Male Sprague Dawley rats were prepared by tightly ligating the left lumbar 5th and 6th spinal nerves and implantation of a chronic lumbar intrathecal catheter for drug administration. The mechanical allodynia was measured by applying von Frey filaments ipsilateral to the lesioned hind paw. And the thresholds for paw withdrawal assessed. In study 1, either R-PIA (0.5, 1 and 2microgram) or saline were administered intrathecally for the examination of the antiallodynic effect of R-PIA. In study 2, glibenclamide (2, 5, 10 and 20 nM) was administered intrathecally 5 min prior to an R-PIA injection for investigation of the reversal of the antiallodynic effects of R-PIA.

RESULTS:

The antiallodynic effect of R-PIA was produced in a dose dependent manner. In study 1, the paw withdrawal threshold was significantly increased with 2microgram R-PIA (P < 0.05). In study 2, the paw withdrawal threshold with 2microgram R-PIA was significantly decreased almost dose dependently by intrathecal pretreatment of 5, 10 and 20 nM glibenclamide (P < 0.05).

CONCLUSIONS:

These results demonstrated that an intrathecal injection of ATP-sensitive potassium channel blockers prior to an intrathecal injection of adenosine A1 receptors agonist had an antagonistic effect on R-PIA induced antiallodynia. The results suggest that the mechanism of mechanical antiallodynia, as induced by an intrathecal injection of R-PIA, may involve the ATP-sensitive potassium channel at both the spinal and supraspinal level in a rat nerve ligation injury model.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Potasio / Nervios Espinales / Inyecciones Espinales / Canales de Potasio / Adenosina / Gliburida / Ratas Sprague-Dawley / Receptores Purinérgicos P1 / Bloqueadores de los Canales de Potasio / Receptor de Adenosina A1 Tipo de estudio: Estudio diagnóstico Límite: Animales / Humanos / Masculino Idioma: Coreano Revista: The Korean Journal of Pain Año: 2005 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Potasio / Nervios Espinales / Inyecciones Espinales / Canales de Potasio / Adenosina / Gliburida / Ratas Sprague-Dawley / Receptores Purinérgicos P1 / Bloqueadores de los Canales de Potasio / Receptor de Adenosina A1 Tipo de estudio: Estudio diagnóstico Límite: Animales / Humanos / Masculino Idioma: Coreano Revista: The Korean Journal of Pain Año: 2005 Tipo del documento: Artículo