The Effects of Growth Factors on Motility of Cultured Human Dermal Microvascular Endothelial Cell

ABSTRACT

Cell migration is essential for many important biological events, including embryonic development, wound healing, inflammatory response, and tumor metastasis. As a result of endothelial cell migration, angiogenesis is very important factor in embryogenesis, wound healing, tumor development and flap survival. Angiogenesis is dependent on endothelial cell proliferation, migration and motility is one of the most essential for many important biological events. The speed of cell migration is regulated by extension, attachment, detachment of cell membrane and adhesiveness of cell to extracellular matrix. Growth factors such as FGF, TGF, VEGF is well known to play a major roles in the migration of endothealial cells. This study was designed to compare the motilities of human dermal microvascular endothelial cell(HDMEC) in growth factors such as bFGF, TGF-beta1 and VEGF. The motility of cultured HDMEC was compared using a video-microscopy system that was developed in combination with a self-designed CO2 mini- incubator. To determine migration speed, cells were viewed with a 4 phase-contrast lens and video recored. Images were captured using a color CCD camera and saved in 8-bit full-color mode. Experimental groups were divided into four groups group I(with a Control, HDMEC only), group II(HDMEC with bFGF), group III (HDMEC with TGF-beta1), group IV(HDMEC with VEGF). At the concentration of 1ng/ml(bFGF), 1ng/ml(TGF-beta1), and 10ng/ml(VEGF) as the most effective dose for cell migration through preliminary study, the speed of migration are 8.736+/-0.948micrometer/hr, 9.869+/-1.904micrometer/ hr, 10.293+/-1.612micrometer/hr, respectively. These data shows that groups with growth factor accelerate the HDMEC migration than a control group, and the VEGF is most effective growth factor in the HDMEC migration than bFGF and TGF-beta1.