Subcellular Ca2+ Mobilization in Gastric Smooth Muscle Contraction / 대한마취과학회지
Korean Journal of Anesthesiology
;
: 101-106, 2002.
Artículo
en Coreano
| WPRIM
| ID: wpr-215939
ABSTRACT
BACKGROUND:
The stomach can be generally classified anatomically into three parts; fundus, corpus, and antrum. It has not been well demonstrated how the three regions contribute to specified gastric motility. In the present study, the regional differences on contractile response and intracellular Ca2+ levels ([Ca2+]i) were investigated in a mouse gastric muscle.METHODS:
An isometrical contraction was measured with a computerized physiograph, and [Ca2+]i was measured with fura-PE3/AM, a fluorescent Ca2+ indicator in gastric smooth muscle from mice.RESULTS:
Carbachol (CCh), a potent muscarinic receptor agonist, generated rhythmic contractions in a dose dependent manner, superimposed on tonic components in the antral muscle. Whereas similar contractile responses to CCh was obtained in the antrum, CCh evoked tonic components predominantly. CCh increased [Ca2+]i in a dose dependent manner in both the antral and fundic smooth muscle. However, the increment of [Ca2+]i in the fundus was greater than that of the antrum. Verapamil (10nM), a l-type Ca2+ channel blocker, inhibited completely the contraction and [Ca2+]i induced by CCh in the antral strips, whereas the responses in the fundus showed a resistance to verapamil.CONCLUSIONS:
These results suggest that muscarinic stimulation has a regional difference on muscle contractility and [Ca2+]i, which is mediated by differences of Ca2+ movement in mouse gastric muscle.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Estómago
/
Carbacol
/
Verapamilo
/
Receptores Muscarínicos
/
Músculo Liso
Límite:
Animales
Idioma:
Coreano
Revista:
Korean Journal of Anesthesiology
Año:
2002
Tipo del documento:
Artículo
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