Comparison of 3 Automated Immunoassays for Detection of Anti-Hepatitis A Virus Immunoglobulin M in a Tertiary Care Hospital
Annals of Laboratory Medicine
;
: 121-124, 2013.
Artículo
en Inglés
| WPRIM
| ID: wpr-216012
ABSTRACT
Three automated immunoassay kits for anti-Hepatitis A Virus (HAV) IgM-Architect, (Abbott Laboratories, USA), Elecsys (Roche Diagnostics, Germany), and ADVIA Centaur (Siemens Healthcare Diagnostics Inc., USA)-were compared. We included 178 consecutive samples, for which an anti-HAV IgM test was requested at Seoul National University Hospital from September 2009 to January 2010. Reviewing of medical records, reverse transcription (RT)-PCR for HAV RNA, or total anti-HAV assay were performed on 16 (9.0%) samples with discrepant results. The percent agreements (kappas) of the Architect and ADVIA Centaur, Architect and Elecsys, and ADVIA Centaur and Elecsys kits were 96.6% (0.91), 96.6% (0.92), and 97.8% (0.94), respectively. Eight out of 16 discrepant samples showed gray-zone values in Architect but were nonreactive in the others. Slightly earlier seroconversion was suspected in Elecsys. The 3 assays showed comparable performances with excellent agreements in a tertiary care hospital setting.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Juego de Reactivos para Diagnóstico
/
Atención Terciaria de Salud
/
Inmunoglobulina M
/
ARN Viral
/
Inmunoensayo
/
Estudios Retrospectivos
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Virus de la Hepatitis A
/
Hepatitis B
/
Hospitales Universitarios
Tipo de estudio:
Estudio diagnóstico
/
Estudio observacional
Límite:
Humanos
Idioma:
Inglés
Revista:
Annals of Laboratory Medicine
Año:
2013
Tipo del documento:
Artículo
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