Usefulness of Immunohistochemistry for Microsatellite Instability Screening in Gastric Cancer
Gut and Liver
;
: 629-635, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-216107
ABSTRACT
BACKGROUND/AIMS:
The usefulness of immunohistochemistry to screen for the microsatellite instability (MSI) phenotype in gastric cancer remains unclear. Moreover, the prognostic value of MSI phenotypes in gastric cancer has been debated.METHODS:
The clinicopathologic parameters and survival outcomes of 203 MSI-high (MSI-H) and 261 microsatellite-stable (MSS) advanced gastric cancers (AGCs) were compared. Next, we compared the immunohistochemistry results for hMLH1 and hMSH2 with those of a polymerase chain reaction (PCR)-based method. Kaplan-Meier curves and a Cox proportional hazard regression model were used to conduct survival analyses.RESULTS:
The MSI-H AGCs were correlated with older age (p<0.001), female gender (p=0.018), distal location (p<0.001), larger size (p=0.016), and intestinal type (p<0.001). Multivariate analysis revealed that the MSI-H phenotype was an independent favorable factor that was related to overall survival in patients with AGC (p<0.001). Compared with the PCR-based analysis, immunohistochemistry exhibited high sensitivity (91.1%) and specificity (98.5%) in the detection of MSI phenotypes.CONCLUSIONS:
MSI-H gastric cancers have distinct clinicopathologic features and better prognoses, which suggests the necessity of MSI analysis in gastric cancer. Immunohistochemistry can be a useful and reliable screening method in the assessment of MSI status in gastric cancer.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fenotipo
/
Pronóstico
/
Neoplasias Gástricas
/
Inmunohistoquímica
/
Modelos de Riesgos Proporcionales
/
Factores Sexuales
/
Reacción en Cadena de la Polimerasa
/
Valor Predictivo de las Pruebas
/
Sensibilidad y Especificidad
/
Inestabilidad de Microsatélites
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
/
Estudio de tamizaje
Límite:
Adulto
/
Anciano
/
Aged80
/
Femenino
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Gut and Liver
Año:
2015
Tipo del documento:
Artículo
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