Effects of Ascorbic Acid on Keratinocyte and Epidermalization of Skin
Annals of Dermatology
;
: 45-51, 2004.
Artículo
en Inglés
| WPRIM
| ID: wpr-216204
ABSTRACT
BACKGROUND:
There are different models of skin substitutes, but no skin substitutes have the characteristics of native skin. It was reported that the incubation of skin substitutes in medium containing ascorbic acid extends cellular viability and promotes formation of an epidermal barrier in vitro.OBJECTIVE:
The purpose of this study is to observe the effects of ascorbic acid on the proliferation of keratinocytes and on the reconstruction of epidermis. MATERIALS ANDMETHODS:
Normal human keratinocytes and fibroblasts were isolated and used for culturing living skin equivalent (LSE).RESULTS:
When ascorbic acid was added, the expression of p63 and a6 integrin was definitely increased compared to control models. In addition, ascorbic acid increased the proliferation of normal human keratinocytes at a dose dependent manner. Especially, ascorbic acid induced the phosphorylation of ERK and up-regulation of EGF-RCONCLUSION:
Results suggest that ascorbic acid is essential in the control of keratinocyte proliferation and basement membrane formation. Ascorbic acid-related keratinocytes proliferation is seemed to be mediated by ERK phosphorylation and EGF-R up-regulation.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fosforilación
/
Ácido Ascórbico
/
Piel
/
Membrana Basal
/
Queratinocitos
/
Regulación hacia Arriba
/
Piel Artificial
/
Epidermis
/
Fibroblastos
Límite:
Humanos
Idioma:
Inglés
Revista:
Annals of Dermatology
Año:
2004
Tipo del documento:
Artículo
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