Proteomic Profiling of Serum from Patients with Tuberculosis
Annals of Laboratory Medicine
;
: 345-353, 2014.
Artículo
en Inglés
| WPRIM
| ID: wpr-216390
ABSTRACT
BACKGROUND:
Effective treatment and monitoring of tuberculosis (TB) requires biomarkers that can be easily evaluated in blood samples. The aim of this study was to analyze the serum proteome of patients with TB and to identify protein biomarkers for TB.METHODS:
Serum samples from 26 TB patients and 31 controls were analyzed by using nano-flow ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry in data-independent mode, and protein and peptide amounts were calculated by using a label-free quantitative approach. The generated data were analyzed by using principal component analysis and partial least squares discriminant analysis, a multivariate statistical method.RESULTS:
Of more than 500 proteins identified, alpha-1-antitrypsin was the most discriminative, which was 4.4 times higher in TB patients than in controls. Peptides from alpha-1-antitrypsin and antithrombin III increased in TB patients and showed a high variable importance in the projection scores and coefficient in partial least square discriminant analysis.CONCLUSIONS:
Sera from patients with TB had higher alpha-1-antitrypsin levels than sera from control participants. Alpha-1-antitrypsin levels may aid in the diagnosis of TB.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Tuberculosis
/
Biomarcadores
/
Análisis Discriminante
/
Alfa 1-Antitripsina
/
Antitrombina III
/
Análisis Multivariante
/
Cromatografía Líquida de Alta Presión
/
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
/
Proteoma
/
Proteómica
Tipo de estudio:
Estudio pronóstico
Límite:
Adulto
/
Anciano
/
Femenino
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Annals of Laboratory Medicine
Año:
2014
Tipo del documento:
Artículo
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