Evaluation of KRAS let-7 lcs6 Polymorphism in Korean Populations by Pyrosequencing / 체질인류학회지
Korean Journal of Physical Anthropology
;
: 131-136, 2012.
Artículo
en Inglés
| WPRIM
| ID: wpr-216934
ABSTRACT
A single nucleotide polymorphism (SNP) in the 3'-untranslated region of the KRAS oncogene (rs61764370) was recently reported to act as a genetic marker for increased risk of developing human cancers. The frequency of the variant allele is 5.8% in world population, however, there is no study in Korean population with large cases. In present study, we evaluated the KRAS let-7 lcs6 SNP in Korean population for the first time. Genotyping was performed in 178 randomized subjects. DNA was extracted and SNP was evaluated by using PCR and pyrosequencing to identify the T or G allele of the KRAS let-7 lcs6 SNP. As a result, only T allele was found in all subjects and quantitative sequencing result showed that the proportion of G allele was extremely low. This study confirmed that KRAS let-7 lcs6 have only one allele system in Korean population by sensitive and quantitative pyrosequencing for the first time. Its clinical significance should be studied further.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oncogenes
/
ADN
/
Marcadores Genéticos
/
Reacción en Cadena de la Polimerasa
/
Polimorfismo de Nucleótido Simple
/
Alelos
Tipo de estudio:
Ensayo Clínico Controlado
Límite:
Humanos
Idioma:
Inglés
Revista:
Korean Journal of Physical Anthropology
Año:
2012
Tipo del documento:
Artículo
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