Alterations in NK Cell Cytotoxicity Induced by Peripheral Nerve Injury in Mice / 대한마취과학회지
Korean Journal of Anesthesiology
;
: 219-223, 2007.
Artículo
en Coreano
| WPRIM
| ID: wpr-218005
ABSTRACT
BACKGROUND:
Chronic pain is often associated with changes in the immune responses, which highlights the need for the aggressive pain control to obtain a better prognosis. This study examined splenic NK cell cytotoxicity in an attempt to assess the possible changes in the immune function under chronic neuropathic pain after a partial transsection of the sciatic nerve.METHODS:
After confirming tactile allodynia in response to the von Frey filament, a modified lactate dehydrogenase (LDH) release assay was used to determine the cytotoxic activity of splenic NK cells on the YAC-1 cell line in C3H/HeN (H-2k) mice (n = 6). NK cells as effector cells were mixed with YAC-1 cells as target cells (1 x 10(4)/100microliter), resulting in an effector-target ratio of 1 25, 1 50, 1 100 in the culture medium.RESULTS:
At 1 and 2 weeks after the nerve injury, all the subjects showed significant mechanical sensitivity compared with those observed before surgery. The percentage of NK cell cytotoxicity of the neuropathic mice increased significantly 1 week after the nerve injury but decreased within 2 weeks compared with the normal mice.CONCLUSIONS:
In terms of the altered NK cell cytotoxicity, neuropathic pain can cause changes in the normal performance of the immune function.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Nervios Periféricos
/
Pronóstico
/
Nervio Ciático
/
Pruebas Inmunológicas de Citotoxicidad
/
Células Asesinas Naturales
/
Línea Celular
/
Dolor Crónico
/
Traumatismos de los Nervios Periféricos
/
Hiperalgesia
/
Sistema Inmunológico
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Coreano
Revista:
Korean Journal of Anesthesiology
Año:
2007
Tipo del documento:
Artículo
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