Serotype- and serogroup-specific detection of African horsesickness virus using phage displayed chicken scFvs for indirect double antibody sandwich ELISAs
Journal of Veterinary Science
;
: 95-98, 2013.
Artículo
en Inglés
| WPRIM
| ID: wpr-219412
ABSTRACT
There is an ongoing need for standardized, easily renewable immunoreagents for detecting African horsesickness virus (AHSV). Two phage displayed single-chain variable fragment (scFv) antibodies, selected from a semi-synthetic chicken antibody library, were used to develop double antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISAs) to detect AHSV. In the DAS-ELISAs, the scFv previously selected with directly immobilized AHSV-3 functioned as a serotype-specific reagent that recognized only AHSV-3. In contrast, the one selected with AHSV-8 captured by IgG against AHSV-3 recognized all nine AHSV serotypes but not the Bryanston strain of equine encephalosis virus. Serving as evidence for its serogroup-specificity. These two scFvs can help to rapidly confirm the presence of AHSV while additional serotype-specific scFvs may simplify AHSV serotyping.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Células Vero
/
Inmunoglobulina G
/
Ensayo de Inmunoadsorción Enzimática
/
Pruebas Serológicas
/
Serotipificación
/
Chlorocebus aethiops
/
Pollos
/
Biblioteca de Péptidos
/
Virus de la Enfermedad Equina Africana
/
Anticuerpos Inmovilizados
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Journal of Veterinary Science
Año:
2013
Tipo del documento:
Artículo
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