Metacercarial proteins interacting with WD40-repeat protein of Clonorchis sinensis
The Korean Journal of Parasitology
;
: 229-232, 2007.
Artículo
en Inglés
| WPRIM
| ID: wpr-219737
ABSTRACT
The WD40-repeat proteins serve as a platform coordinating partner proteins and are involved in a range of regulatory cellular functions. A WD40-repeat protein (CsWD1) of Clonorchis sinensis previously cloned is expressed stage-specifically in the tegumental syncytium of C. sinensis metacercariae. In the present study, interacting proteins with the CsWD1 protein was purified by immunoprecipitation and 2 dimension gel electrophoresis from the C. sinensis metacercaria soluble extract, and tryptic peptides were analyzed by LC/ESI-MS. Putative partner proteins were annotated to be actin-2, glyceraldehyde-3-phosphate dehydrogenase, and hypothetical and unmanned proteins. The CsWD1 protein was predicted to contain 3 conserved actin-interacting residues on its functional surface. With these results, the CsWD1 protein is suggested to be an actin-interacting protein of C. sinensis.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Inmunoglobulina G
/
Anticuerpos Antihelmínticos
/
Electroforesis en Gel Bidimensional
/
Proteínas del Helminto
/
Clonorchis sinensis
/
Concentración de Iones de Hidrógeno
/
Proteínas de Microfilamentos
Límite:
Animales
Idioma:
Inglés
Revista:
The Korean Journal of Parasitology
Año:
2007
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS