Your browser doesn't support javascript.
loading
Protective Effect of Heme Oxygenase-1 on High Glucose-Induced Pancreatic beta-Cell Injury
Diabetes & Metabolism Journal ; : 469-479, 2011.
Artículo en Inglés | WPRIM | ID: wpr-22257
ABSTRACT

BACKGROUND:

Glucose toxicity that is caused by chronic exposure to a high glucose concentration leads to islet dysfunction and induces apoptosis in pancreatic beta-cells. Heme oxygenase-1 (HO-1) has been identified as an anti-apoptotic and cytoprotective gene. The purpose of this study is to investigate whether HO-1 up-regulation when using metalloprotophyrin (cobalt protoporphyrin, CoPP) could protect pancreatic beta-cells from high glucose-induced apoptosis.

METHODS:

Reverse transcription-polymerase chain reaction was performed to analyze the CoPP-induced mRNA expression of HO-1. Cell viability of INS-1 cells cultured in the presence of CoPP was examined by acridine orange/propidium iodide staining. The generation of intracellular reactive oxygen species (ROS) was measured using flow cytometry. Glucose stimulated insulin secretion (GSIS) was determined following incubation with CoPP in different glucose concentrations.

RESULTS:

CoPP increased HO-1 mRNA expression in both a dose- and time-dependent manner. Overexpression of HO-1 inhibited caspase-3, and the number of dead cells in the presence of CoPP was significantly decreased when exposed to high glucose conditions (HG). CoPP also decreased the generation of intracellular ROS by 50% during 72 hours of culture with HG. However, decreased GSIS was not recovered even in the presence of CoPP.

CONCLUSION:

Our data suggest that CoPP-induced HO-1 up-regulation results in protection from high glucose-induced apoptosis in INS-1 cells; however, glucose stimulated insulin secretion is not restored.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Protoporfirinas / ARN Mensajero / Regulación hacia Arriba / Supervivencia Celular / Especies Reactivas de Oxígeno / Apoptosis / Diabetes Mellitus / Hemo-Oxigenasa 1 / Caspasa 3 / Citometría de Flujo Idioma: Inglés Revista: Diabetes & Metabolism Journal Año: 2011 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Protoporfirinas / ARN Mensajero / Regulación hacia Arriba / Supervivencia Celular / Especies Reactivas de Oxígeno / Apoptosis / Diabetes Mellitus / Hemo-Oxigenasa 1 / Caspasa 3 / Citometría de Flujo Idioma: Inglés Revista: Diabetes & Metabolism Journal Año: 2011 Tipo del documento: Artículo