Fusobacterium nucleatum GroEL signaling via Toll-like receptor 4 in human microvascular endothelial cells
International Journal of Oral Biology
;
: 130-136, 2012.
Artículo
en Inglés
| WPRIM
| ID: wpr-222608
ABSTRACT
The GroEL heat-shock protein from Fusobacterium nucleatum, a periodontopathogen, activates risk factors for atherosclerosis in human microvascular endothelial cells (HMEC-1) and ApoE-/- mice. In this study, we analyzed the signaling pathways by which F. nucleatum GroEL induces the proinflammatory factors in HMEC-1 cells known to be risk factors associated with the development of atherosclerosis and identified the cellular receptor used by GroEL. The MAPK and NF-kappaB signaling pathways were found to be activated by GroEL to induce the expression of interleukin-8 (IL-8), monocyte chemoattractant protein 1 (MCP-1), intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), E-selectin, and tissue factor (TF). These effects were inhibited by a TLR4 knockdown. Our results thus indicate that TLR4 is a key receptor that mediates the interaction of F. nucleatum GroEL with HMEC-1 cells and subsequently induces an inflammatory response via the MAPK and NF-kappaB pathways.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Periodontitis
/
Tromboplastina
/
Factores de Riesgo
/
Interleucina-8
/
FN-kappa B
/
Fusobacterium nucleatum
/
Molécula 1 de Adhesión Intercelular
/
Molécula 1 de Adhesión Celular Vascular
/
Quimiocina CCL2
/
Selectina E
Tipo de estudio:
Estudio de etiología
/
Estudio pronóstico
/
Factores de riesgo
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
International Journal of Oral Biology
Año:
2012
Tipo del documento:
Artículo
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