The expression and interaction of HLA-G and IL-10 in cervical cancer / 대한산부인과학회지
Korean Journal of Obstetrics and Gynecology
;
: 126-134, 2007.
Artículo
en Coreano
| WPRIM
| ID: wpr-224169
ABSTRACT
OBJECTIVES:
To determine the expression of HLA-G and IL-10 and their correlation in tissue of cervical cancer, and to investigate the relationship between their expression and clinicopathologic factors in patients with cervical cancer.METHODS:
Tissue samples were obtained from 40 patients diagnosed with cervical cancer and 15 patients with normal cervix for control from Oct. 2004 to Oct. 2005. Quantitative real-time RT-PCR for HLA-G mRNA and semi-quantitative RT-PCR for IL-10 mRNA were used. And proteins of HLA-G and IL-10 were detected by Western blot analysis.RESULTS:
Both HLA-G and IL-10 mRNA expression in cervical cancer tissue were higher than normal control, which was statistically significant (P<0.001, P<0.001). The proteins levels of HLA-G and IL-10 in cancer group were also significantly higher than control (P<0.001, P=0.041). The mRNA expression of HLA-G tended to be correlated with IL-10 expression (P=0.061), although it was not statistically significant. Among clinicopathologic factors of cervical cancer, there was inverse relationship between FIGO stage and mRNA value of HLA-G (P=0.045).CONCLUSIONS:
The mRNA and protein expression of HLA-G and IL-10 in cervical cancer were much higher than in controls. These results showed that HLA-G and IL-10 might have an important role of tumorigenesis in patients with cervical cancer. The levels of HLA-G and IL-10 seem to be correlated although it was not statistically significant. High HLA-G mRNA expression could be related in early tumorigenesis since it was associated with early stage cervical cancer.
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
ARN Mensajero
/
Neoplasias del Cuello Uterino
/
Cuello del Útero
/
Western Blotting
/
Interleucina-10
/
Antígenos HLA-G
/
Carcinogénesis
Límite:
Femenino
/
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Obstetrics and Gynecology
Año:
2007
Tipo del documento:
Artículo
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