Evaluation of Biologic Phenotype by Midkine Gene Expression in Gastric Cancer as a Target for Biotherapy / 대한암학회지
Journal of the Korean Cancer Association
;
: 69-80, 1997.
Artículo
en Coreano
| WPRIM
| ID: wpr-224326
ABSTRACT
PURPOSE:
We studied biological phenotypes of gastric cancer cell lines based on a novel heparin-binding growth/differentiation factor, midkine (MK) expression. MATERIALS ANDMETHODS:
Nine gastric cancer cell lines and 25 gastric cancer tissues were tested for MK expression by Northern blot analysis. Soft agar assay for in vitro tumorigenesis, cross- feeding assay for paracrine angiogenic activity, ELISA for uPA and PAI-1 measurement were performed.RESULTS:
MK expression was found in 67% (6/9) of the gastric cancer cell lines, and 56% (14/25) of the primary gastric cancer tissues. Gastric cancer cell lines with MK expression were more tumorigenic in soft agar assay and endothelial cell growth stimulatory in cross-feeding assay than cells which did not express MK. However, urokinase-type plasminogen activator (uPA) expression did not correlate with MK expression. Growth of MK expressing cells was inhibited by a heparin-binding blocking agent, pentosan polysulfate (PPS). In cancer tissues, MK expression correlated with tumor size, suggesting in vivo autocrine and paracrine activity.CONCLUSION:
Gastric cancer cells with increased MK gene expression showed increased tumorigenic and angiogenic activity. Therefore, this proliferation promoting activity of MK can be targeted by an anti-heparin binding agent as a biotherapy model in gastric cancer treatment.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Poliéster Pentosan Sulfúrico
/
Fenotipo
/
Neoplasias Gástricas
/
Terapia Biológica
/
Ensayo de Inmunoadsorción Enzimática
/
Activador de Plasminógeno de Tipo Uroquinasa
/
Expresión Génica
/
Línea Celular
/
Northern Blotting
/
Inhibidor 1 de Activador Plasminogénico
Tipo de estudio:
Estudio pronóstico
Idioma:
Coreano
Revista:
Journal of the Korean Cancer Association
Año:
1997
Tipo del documento:
Artículo
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