Comparison of Traditional and Reverse Syphilis Screening Algorithms in Medical Health Checkups
Annals of Laboratory Medicine
;
: 511-515, 2017.
Artículo
en Inglés
| WPRIM
| ID: wpr-224344
ABSTRACT
BACKGROUND:
The syphilis diagnostic algorithms applied in different countries vary significantly depending on the local syphilis epidemiology and other considerations, including the expected workload, the need for automation in the laboratory and budget factors. This study was performed to investigate the efficacy of traditional and reverse syphilis diagnostic algorithms during general health checkups.METHODS:
In total, 1,000 blood specimens were obtained from 908 men and 92 women during their regular health checkups. Traditional screening and reverse screening were applied to the same specimens using automatic rapid plasma regain (RPR) and Treponema pallidum latex agglutination (TPLA) tests, respectively. Specimens that were reverse algorithm (TPLA) reactive, were subjected to a second treponemal test performed by using the chemiluminescent microparticle immunoassay (CMIA).RESULTS:
Of the 1,000 specimens tested, 68 (6.8%) were reactive by reverse screening (TPLA) compared with 11 (1.1%) by traditional screening (RPR). The traditional algorithm failed to detect 48 specimens [TPLA(+)/RPR(−)/CMIA(+)]. The median TPLA cutoff index (COI) was higher in CMIA-reactive cases than in CMIA-nonreactive cases (90.5 vs 12.5 U).CONCLUSIONS:
The reverse screening algorithm could detect the subjects with possible latent syphilis who were not detected by the traditional algorithm. Those individuals could be provided with opportunities for evaluating syphilis during their health checkups. The COI values of the initial TPLA test may be helpful in excluding false-positive TPLA test results in the reverse algorithm.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plasma
/
Automatización
/
Sífilis Latente
/
Treponema pallidum
/
Presupuestos
/
Inmunoensayo
/
Sífilis
/
Tamizaje Masivo
/
Epidemiología
/
Aglutinación
Tipo de estudio:
Estudio diagnóstico
/
Evaluación Económica en Salud
/
Estudio pronóstico
/
Estudio de tamizaje
Límite:
Femenino
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Annals of Laboratory Medicine
Año:
2017
Tipo del documento:
Artículo
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