Sperm DNA fragmentation and sex chromosome aneuploidy after swim-up versus density gradient centrifugation / 대한생식의학회지
Clinical and Experimental Reproductive Medicine
;
: 201-206, 2017.
Artículo
en Inglés
| WPRIM
| ID: wpr-226344
ABSTRACT
OBJECTIVE:
The aim of this study was to compare the efficacy of swim-up and density gradient centrifugation (DGC) for reducing the amount of sperm with fragmented DNA, sex chromosome aneuploidy, and abnormal chromatin structure.METHODS:
Semen samples were obtained from 18 healthy male partners who attended infertility clinics for infertility investigations and were processed with swim-up and DGC. The percentages of sperm cells with fragmented DNA measured by the sperm chromatin dispersion test, normal sex chromosomes assessed by fluorescence in situ hybridization, and abnormal chromatin structure identified by toluidine blue staining were examined.RESULTS:
The percentage of sperm cells with fragmented DNA was significantly lower in the swim-up fraction (9.7%, p=0.001) than in the unprocessed fraction (27.0%), but not in the DGC fraction (27.8%, p=0.098). The percentage of sperm cells with normal X or Y chromosomes was comparable in the three fractions. The percentage of sperm cells with abnormal chromatin structure significantly decreased after DGC (from 15.7% to 10.3%, p=0.002). The swim-up method also tended to reduce the percentage of sperm cells with abnormal chromatin structure, but the difference was not significant (from 15.7% to 11.6%, p=0.316).CONCLUSION:
The swim-up method is superior for enriching genetically competent sperm.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Semen
/
Cromosomas Sexuales
/
Espermatozoides
/
Cloruro de Tolonio
/
Cromosoma Y
/
ADN
/
Cromatina
/
Centrifugación por Gradiente de Densidad
/
Hibridación in Situ
/
Fragmentación del ADN
Límite:
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Clinical and Experimental Reproductive Medicine
Año:
2017
Tipo del documento:
Artículo
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