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Genetic Diversity of Dematiaceous Fungi Using Random Amplified Polymorphic DNA / 대한의진균학회지
Korean Journal of Medical Mycology ; : 7-15, 2003.
Artículo en Coreano | WPRIM | ID: wpr-226744
ABSTRACT

BACKGROUND:

There are three kinds of diseases caused by dematiaceous fungi chromoblastomycosis, phaeohyphomycosis, and eumycotic mycetoma. The dematiaceous fungi have been identified and classified by morphological, biochemical and physiological tests. Recently molecular analysis has been introduced to the field of medical mycology.

OBJECTIVE:

We investigated the genetic diversity of dematiaceous fungi using random amplified polymorphic DNA (RAPD).

METHODS:

The dematiaceous fungal strains studied were eight clinical isolates of chromoblastomycosis and phaeohyphomycosis agents (3 strains of Fonsecaea pedrosoi, 2 strains of Exophiala dermatitidis, 1 strain of Exophiala jeanselmei, 1 strain of Phialophora verrucosa, 1 strain of Rhinocladiella aquaspersa) and 4 standard strains (F. pedrosoi IFM 4889, E. dermatitidis IFM 4828, P. verrucosa IFM 4928, R. aquaspersa IFM 4930). Total twelve strains of dematiaceous fungi were cultured on Sabouraud's dextrose broth and their DNA were extracted by bead-beating method.

RESULTS:

The optimal condition for PCR was template DNA 0.025 mg and annealing temperature 39 degrees C. The RAPD analysis using OPA 10 primer (5'-GTGATCGCAG-3') of Operon kit showed different patterns among dematiaceous fungi. But one clinical isolate of F. pedrosoi showed intra-specific variability.

CONCLUSION:

The RAPD analysis is considered a rapid and reliable method for identification and classification of dematiaceous fungi if the procedure is carefully standardized with adequate primer.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Operón / Phialophora / Variación Genética / Exophiala / ADN / Reacción en Cadena de la Polimerasa / Cromoblastomicosis / Clasificación / Feohifomicosis / Hongos Tipo de estudio: Ensayo Clínico Controlado Idioma: Coreano Revista: Korean Journal of Medical Mycology Año: 2003 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Operón / Phialophora / Variación Genética / Exophiala / ADN / Reacción en Cadena de la Polimerasa / Cromoblastomicosis / Clasificación / Feohifomicosis / Hongos Tipo de estudio: Ensayo Clínico Controlado Idioma: Coreano Revista: Korean Journal of Medical Mycology Año: 2003 Tipo del documento: Artículo