Cronobacter sakazakii Infection Induced Fatal Clinical Sequels Including Meningitis in Neonatal ICR Mice / 한국실험동물학회지
Laboratory Animal Research
;
: 59-62, 2011.
Artículo
en Inglés
| WPRIM
| ID: wpr-227292
ABSTRACT
Cronobacter sakazakii (C. sakazakii), formerly Enterobacter sakazakii, is an emerging pathogen associated with the ingestion of contaminated reconstituted formula that causes serious illnesses such as bacteremia, septicemia, necrotizing enterocolitis, meningitis and death in low-birth-weight preterm neonatal infants. The objective of this study was to develop an animal model for human neonatal C. sakazakii infections. We acquired timed-pregnant ICR mice and allowed them to give birth naturally. On postnatal day 3.5, each pup was administered orally a total dose of approximately 107 CFU C. sakazakii strain 3439. Mice were observed twice daily for morbidity and mortality. At postnatal day 10.5, the remaining pups were euthanized, and brain, liver, and cecum were excised and analyzed for the presence of C. sakazakii. C. sakazakii was isolated from cecum and other tissues in inoculated mice. In the tissues of C. sakazakii infected mice, meningitis and gliosis were detected in brain. In this study, we confirmed the neonatal ICR mice may be used a very effective animal model for human neonatal C. sakazakii infections.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Esguinces y Distensiones
/
Encéfalo
/
Ciego
/
Bacteriemia
/
Sepsis
/
Enterocolitis Necrotizante
/
Modelos Animales
/
Parto
/
Cronobacter sakazakii
/
Ingestión de Alimentos
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Laboratory Animal Research
Año:
2011
Tipo del documento:
Artículo
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