Presence of E-cadherin in Cultured Amnionic Cells / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 2690-2694, 1999.
Artículo
en Coreano
| WPRIM
| ID: wpr-228947
ABSTRACT
OBJECTIVES:
Cell adhesion molecules may play a role in integrating amnionic membrane. The objective of this study was to prove E-cadherin mRNA and proteins in cultured human amnionic cells.METHODS:
We cultured amnionic cells from 4 women undergoing cesarean section without labor. E-cadherin was determined by immunohistochemistry and Western ligend blotting. To demonstrate E-cadherin mRNA, reverse transcription-polymerase chain reaction was performed.RESULTS:
On immunohistochemistry, E-cadherin was abundantly showed on the cytoplasm of the cells. Western ligend blotting showed clear 120 kDa bands on four specimens, and relatively weak band on one specimen. Reverse transcription-polymerase chain reaction showed 432 BP bands.CONCLUSION:
We proved E-cadherin and its mRNA by immunohistochemistry, Western ligend blotting and reverse transcription-polymerase chain reaction in cultured human amnionic cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
ARN Mensajero
/
Inmunohistoquímica
/
Cadherinas
/
Moléculas de Adhesión Celular
/
Cesárea
/
Citoplasma
/
Amnios
/
Membranas
Límite:
Femenino
/
Humanos
/
Embarazo
Idioma:
Coreano
Revista:
Korean Journal of Obstetrics and Gynecology
Año:
1999
Tipo del documento:
Artículo
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