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High-performance liquid chromatographic determination of urinary trans, trans-muconic acid excreted by workers occupationally exposed to benzene / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences ; (12): 292-296, 2006.
Artículo en Inglés | WPRIM | ID: wpr-229686
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the relationship between trans, trans-muconic acid (ttMA) as benzene metabolite of occupational workers and benzene concentration in air.</p><p><b>METHODS</b>A rapid and sensitive high-performance liquid chromatography was developed to determine the level of urinary ttMA. ttMA was extrated from urinary samples in liquid-liquid phase a ODS (2) (5u) column (phi 4.6 mm x 150 mm) and detected at wavelength 264 nm in a UV detector using vanillic acid as an internal standard. The mobile phase was acetaticacid/tetrahydrofuran/methanol/water (v/v, 121087). The method was validated with 56 urine samples collected from occupationally benzene-exposed individuals.</p><p><b>RESULTS</b>A correlation coefficient (r = 0.9963) was found for ttMA ranging 0.10-10.00 microg/mL. The limit of detection was 0.10 microg/mL. The recovery and reproducibility were generally over 90%. There was a positive correlation between ttMA and benzene level in air. The equation was Y = 0.859 + 0.108C (before work, r = 0.6200) or Y = 1.980 + 0.179C (after work, r = 0.7930).</p><p><b>CONCLUSION</b>This method can be used to determine and control the level of urinary ttMA in those who are occupationally exposed to benzene.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Ácido Sórbico / Orina / Benceno / Calibración / Biomarcadores / Monitoreo del Ambiente / Reproducibilidad de los Resultados / Cromatografía Líquida de Alta Presión / Exposición Profesional / Contaminantes Ocupacionales del Aire Límite: Humanos Idioma: Inglés Revista: Biomedical and Environmental Sciences Año: 2006 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Ácido Sórbico / Orina / Benceno / Calibración / Biomarcadores / Monitoreo del Ambiente / Reproducibilidad de los Resultados / Cromatografía Líquida de Alta Presión / Exposición Profesional / Contaminantes Ocupacionales del Aire Límite: Humanos Idioma: Inglés Revista: Biomedical and Environmental Sciences Año: 2006 Tipo del documento: Artículo