The building of chip-based capillary electrophoresis platform and application / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 709-712, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-229837
ABSTRACT
<p><b>OBJECTIVE</b>To detect the point mutations by chip-based capillary electrophoresis and to provide a rapid and sensitive technique detection for beta-thalassemia.</p><p><b>METHODS</b>Multiplex primer-extension reaction was used to amplify the common loci of the samples for beta-thalassemia. The reaction products were detected by the chip-based capillary electrophoresis and the genotypes of the samples were discrened.</p><p><b>RESULTS</b>A system was constructed to detect the point mutations of beta-thalassemia by chip-based capillary electrophoresis, and the technology was ralidated by the patients' samples and the results coincided with those of detection kit.</p><p><b>CONCLUSION</b>Beta-thalassemia can be detected by chip-based capillary electrophoresis rapidly with a small amount of samples. It would be a new detection method of the genetic disorders.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Talasemia beta
/
Electroforesis Capilar
/
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Diagnóstico
/
Genética
/
Enfermedades Genéticas Congénitas
/
Métodos
Tipo de estudio:
Estudio diagnóstico
Límite:
Femenino
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Chinese Journal of Medical Genetics
Año:
2007
Tipo del documento:
Artículo
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