Establishment of systemic lupus erythematosus-like murine model with Sm mimotope / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 191-195, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-230006
ABSTRACT
<p><b>OBJECTIVE</b>To establish systemic lupus erythematosus (SLE) -like murine model by immunizing BALB/C mice with Sm mimotope.</p><p><b>METHODS</b>Sm mimotope was identified by screening a 12-mer random peptide library with monoclonal anti-Smith antibody. Sm mimotope was initially defined with sandwich ELISA, DNA sequencing, and deduced amino acid sequence; and BALB/C mice were subcutaneously injected with mixture phages clones. Sera Sm antibody, anti-double stranded DNA (dsDNA) antibody, and antinuclear antibody (ANA) of mice were detected using direct immunofluorescence; kidney histological changes were examined by HE staining.</p><p><b>RESULTS</b>Five randomly selected peptides were sequenced and the amino acid sequences IR, SQ, and PP were detected in a higher frequency. High-titer IgG autoantibodies of dsDNA, Sm, and ANA in the sera of experiment group were detected by ELISA 28 days after having been immunized by Sm mimotope. Proteinuria was detected 33 days later; immune complex and nephritis were observed in kidney specimens.</p><p><b>CONCLUSION</b>SLE-like murine model can be successfully induced by Sm phage mimotope.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oligopéptidos
/
Autoanticuerpos
/
Sangre
/
Inmunoglobulina G
/
Anticuerpos Antinucleares
/
Imitación Molecular
/
Biblioteca de Péptidos
/
Modelos Animales de Enfermedad
/
Alergia e Inmunología
/
Lupus Eritematoso Sistémico
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Acta Academiae Medicinae Sinicae
Año:
2007
Tipo del documento:
Artículo
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