Expression and role of toll-like receptors in U937 cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 449-453, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-230247
ABSTRACT
The aim of study was to explore the potential application of targeting at Toll-like receptors (TLRs) in the immunotherapy of acute myelocytic leukemia, and to investigate the expression of TLR and the effects of TLR 8 agonist ssRNA40/LyoVec on proliferation, apoptosis and cell cycle of U937 cells. The expression of TLR 1 - 9 in U937 cells was detected by using reverse transcription polymerase chain reaction (RT-PCR) and the expression of TLR 8 was assayed by flow cytometry (FCM). The effect of TLR 8 agonist, ssRNA40/LyoVec, at different concentrations on U937 cells proliferation was evaluated by CCK-8, apoptosis and cell cycle were detected by FCM. The results showed that U937 cells expressed TLR 1 - 9. TLR 8 agonist ssRNA40/LyoVec could inhibit the growth of U937 cells both in time-and dose-dependent manner and the inhibitory rate could reach 70%. It also increased the percentage of cells in G(0)/G(1) phase. There was no significant difference in percentage of apoptotic cells between control and treated groups. It is concluded that TLRs including TLR 1 - 9 express on U937 cells and TLR 8 agonist ssRNA40/LyoVec may be able to inhibit the growth of U937 cells, arrest the cells in G(0)/G(1) phase, but have no effect of promoting apoptosis.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Leucemia Mieloide Aguda
/
Ciclo Celular
/
Apoptosis
/
Células U937
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Proliferación Celular
/
Relación Dosis-Respuesta a Droga
/
Receptores Toll-Like
/
Receptor Toll-Like 8
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2007
Tipo del documento:
Artículo
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