Effect of nimodipine on mechanisms of HL-60 cell apoptosis induced by cytarabine / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 72-75, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-230330
ABSTRACT
The aim was to study the mechanisms of HL-60 cell apoptosis induced by nimodipine (NMDP) and cytarabine (Ara-C). The DNA fragment was detected by agarose gel electrophoresis. The expressions of bcl-2 and bax gene proteins related with apoptosis were investigated by immunohistochemistry. The results showed that HL-60 cell apoptosis rate had been increasing in the experimental groups compared with the control group since culturing 8 hours. The expression of Bcl-2 protein was lower and the expression of Bax protein was higher in the experimental groups than that in the control group, while ratio of bcl-2/bax was lower in the experimental groups than that in the control group. It is concluded that NMDP and Ara-C induce apoptosis of HL-60 cells, and the mechanism of apoptosis induced by them may down-regulate the expression of bcl-2 gene and up-regulate the expression of bax gene. The mechanism of HL-60 cell apoptosis induced by Ara-C and NMDP is probably associated with the down-regulation of Bcl-2 protein expression.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Nimodipina
/
Regulación Neoplásica de la Expresión Génica
/
Apoptosis
/
Células HL-60
/
Resistencia a Antineoplásicos
/
Proteínas Proto-Oncogénicas c-bcl-2
/
Citarabina
/
Sinergismo Farmacológico
/
Proteína bcl-X
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2007
Tipo del documento:
Artículo
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