Prenatal Diagnosis of Duchenne Muscular Dystrophy (DMD) by Multiplex Polymerase Chain Reaction (PCR) with Chorionic Villi / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 2028-2032, 1999.
Artículo
en Coreano
| WPRIM
| ID: wpr-23040
ABSTRACT
OBJECTIVE:
To evaluate the efficacy of prenatal multiplex PCR with chorionic villi, in the case of family history of DMD due to exon deletion.METHODS:
DNA was extracted when cells' size are 1x 106 on T- flask surface area reach to 25 cm2 after 2 weeks from sex confirmation. Average DNA concentration was 50-100 ng and multiplex PCR test was performed from DNA extraction.RESULT:
PCR was done for 17 exons devided into 4 groups. Seventeen exons were all amplified with their right size.CONCLUSIONS:
This method is DNA analysis for prenatal diagnosis of DMD with chorionic villi in the family of DMD. This is useful when preimplantation genetic diagnosis is not available.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Diagnóstico Prenatal
/
ADN
/
Reacción en Cadena de la Polimerasa
/
Exones
/
Corion
/
Vellosidades Coriónicas
/
Diagnóstico Preimplantación
/
Distrofia Muscular de Duchenne
/
Reacción en Cadena de la Polimerasa Multiplex
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Obstetrics and Gynecology
Año:
1999
Tipo del documento:
Artículo
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