Separation and identification of the exosomes derived from a mouse hepatoma carcinoma cell line (H22) and initial investigation of their protein composition / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 437-440, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-230573
ABSTRACT
<p><b>OBJECTIVE</b>To separate and identify the exosomes derived from a mouse hepatoma carcinoma cell line (H22) and to detect their protein composition, and to investigate the possibility of using these exosomes as a kind of tumor vaccine.</p><p><b>METHODS</b>Exosomes were purified by serial ultracentrifugation and sugar density ultracentrifugation, and then they were observed and identified by electron microscopy. Exosomes underwent peptide mass fingerprint and Western blot analyses.</p><p><b>RESULTS</b>H22 cell-derived exosomes were 20-90 nm round or oval vesicles. The exosomes expressed HSP70, ICAM-1, EF-G2, DLC-A, C-myc protein and Vav-2 protein.</p><p><b>CONCLUSION</b>Serial ultracentrifugation and sugar density ultracentrifugation can be used to purify H22 cell-derived exosomes. H22 cell-derived exosomes express a distinct set of proteins involving in and/or relating to antigen presentation (HSP70, ICAM-1), migration (DLC-A), adhesion (ICAM-1), cytoskeleton (EF-G2) and tumour antigens (C-myc, Vav-2). The exosomes have immunogenicity.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Mapeo Peptídico
/
Antígenos de Histocompatibilidad Clase II
/
Carcinoma Hepatocelular
/
Secreciones Corporales
/
Línea Celular Tumoral
/
Alergia e Inmunología
/
Exosomas
/
Neoplasias Hepáticas
/
Metabolismo
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Hepatology
Año:
2007
Tipo del documento:
Artículo
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