Heat-shock protein 27 linked to multi-drug resistance in human hepatic cancer cell lines / 中华肝脏病杂志

ABSTRACT

<p><b>OBJECTIVE</b>A proteomics approach was applied for finding multi-drug resistance (MDR) related proteins in hepatic cancer cell lines.</p><p><b>METHODS</b>Two-dimensional electrophoresis (2-DE) was used to separate the total proteins of vincristine-resistant hepatic cancer cell line HepG2/VCR and its counterpart HepG2. The differential expression proteins between the two cell lines were identified by both MALDI-TOF-MS and ESI-Q-TOF-MS. Heat-shock protein 27 (HSP27), one of the differential expression proteins in the development of MDR of HepG2/VCR, was analyzed by HSP27 antisense oligonucleotides (ASOs).</p><p><b>RESULTS</b>As a high expression protein in HepG2/VCR, HSP27 was identified, and the suppression of HSP27 expression by HSP27 ASO enhanced the vincristine chemosensitivity of HepG2/VCR (P less than 0.05).</p><p><b>CONCLUSION</b>HSP27 is linked to MDR in human hepatic cancer cell line HepG2/VCR.</p>