Expression of eotaxin mRNA and TNF-alpha mRNA in lung tissues of sensitized mice and modulation by anti-inflammatory drugs / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 283-286, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-231066
ABSTRACT
<p><b>OBJECTIVE</b>To establish determination methods of eotaxin mRNA and TNF-alpha mRNA expression in the lung tissue of mice.</p><p><b>METHODS</b>Eotaxin mRNA and TNF-alpha mRNA expressions were determined by semi-quantitative RT-PCR. The functional implications of eotaxin mRNA and TNF-alpha mRNA expression were examined by detecting the numbers of total leucocytes and eosinophils in bronchoalveolar lavage fluid(BALF).</p><p><b>RESULT</b>Eotaxin mRNA and TNF-alpha mRNA expression in lung tissue total numbers of leucocyte and numbers of eosinophil in BALF increased in sensitized mice compared with those in normal mice. Dexamethasone significantly but did not inhibit eotaxin mRNA and TNF-alpha mRNA expressions, and eosinophil infiltration in the lungs of the sensitized mice. A compound preparation of traditional Chinese medicine inhibited eotaxin mRNA and eosinophil infiltration, influenced TNF-alpha mRNA expression.</p><p><b>CONCLUSION</b>Increased eotaxin mRNA expression in lung tissue is associated with eosinophil infiltration in BALF, which indicates that the methods of semi-quantitative RT-PCR may be useful to the study of the mechanism of antiasthmatic medicine.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Fisiología
/
Asma
/
ARN Mensajero
/
Dexametasona
/
Líquido del Lavado Bronquioalveolar
/
Factor de Necrosis Tumoral alfa
/
Quimiocinas CC
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Biología Celular
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Zhejiang University. Medical sciences
Año:
2003
Tipo del documento:
Artículo
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