Your browser doesn't support javascript.
loading
Effects of different methanol feeding strategy on hirudin production in high-density fermentation by recombinant Pichia pastoris / 生物工程学报
Chinese Journal of Biotechnology ; (12): 348-351, 2002.
Artículo en Chino | WPRIM | ID: wpr-231319
ABSTRACT
Four different methanol feeding modes were evaluated in the hirudin production in high-density fermentation by Pichia pastoris. It was difficult to avoid methanol excessive in the broth with the feeding strategy only based on DO level. On the other hand, the fluctuation in methanol concentration was observed with methanol feeding strategy by off-line gas chromatography. However, the stable methanol concentration was perfectly achieved by the on-line monitoring with methanol sensor. The supply of energy was improved by feeding glycerol at a limited rate as well as methanol in the induction phase. Therefore, the high cell dry weight (162 g/L) and high hirudin activity (2.4 x 10(4) ATU/mL or 1.7 g/L) was obtained in the fed-batch fermentation of recombinant Pichia pastoris by methanol-glycerol mixed feeding.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Pichia / Recombinación Genética / Hirudinas / Metanol / Fermentación / Genética / Glicerol / Metabolismo Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2002 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Pichia / Recombinación Genética / Hirudinas / Metanol / Fermentación / Genética / Glicerol / Metabolismo Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2002 Tipo del documento: Artículo