Studies on lipase-catalyzed synthesis of ethylglucoside lactate in non-aqueous phase / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 94-98, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-231365
ABSTRACT
Ethylglucoside lactate, a novel Alpha-Hydroxy Acids Derivative, was synthesized by transesterification in non-aqueous phase using immobilized lipase as biocatalyst. Based on the studies of the factors effecting initial rate and conversion under atmospheric pressure (solvent, acyl donor, different immobilized lipase, substrate concentration, enzyme concentration and temperature), the results show that solvent-free medium using butyllactate as acyl donor is suitable to the ester synthesis. The reaction conditions have been optimized as the following the amount of enzyme = 75 g/L, the ethylglucoside concentration = 0.4 mol/L, 70 degrees C, 200 r/min, 50 h, which the conversion was 71%. A 90% conversion and a 60.7 mmol.L-1.h-1 initial rate can be obtained under reduced pressure, which the conditions are enzyme 75 g/L, ethylglucoside 0.35 mol/L, 65 degrees C, 200 r/min and 40 h. The product purified by extraction and SIO2 chromatography was identified by infrared spectroscopy and 1H NMR.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Especificidad por Sustrato
/
Temperatura
/
Candida
/
Cinética
/
Ácido Láctico
/
Enzimas Inmovilizadas
/
Ésteres
/
Glucósidos
/
Lactatos
/
Lipasa
Tipo de estudio:
Estudio pronóstico
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2002
Tipo del documento:
Artículo
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