Cloning and expression of Buthus martensii Karsch scorpion toxin gene (BmK IT3) in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 106-108, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-231387
ABSTRACT
According to the reported sequence of Buthus martensii Karsch scorpion toxin gene (BmK IT3), we synthesized two primers, which were complementary in a region. By the means of PCR, we got the gene. The gene was fused in expression vector pET-28a, which gave rise to a recombinant plasmid pET(IT3R). Then it was transformed into E. coli BL21 (DE3). With IPTG induction, the gene was efficiently expressed. And the fusion product was soluble.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Venenos de Escorpión
/
Escorpiones
/
Proteínas Recombinantes
/
Expresión Génica
/
Química
/
Clonación Molecular
/
Escherichia coli
/
Genética
/
Isopropil Tiogalactósido
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2002
Tipo del documento:
Artículo
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