Screening, identification and analysis of platelet differential functional proteins in patients with coronary heart disease of blood-stasis pattern / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 467-473, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-231499
ABSTRACT
<p><b>OBJECTIVE</b>To seek the key platelet functional proteins (PFPs) for the occurrence of blood-stasis pattern (BP) in patients with coronary heart disease (CHD).</p><p><b>METHODS</b>Peripheral blood platelet protein of 22 patients and 24 healthy person (for control) were extracted respectively in 4 batches for carrying 4 times of the test out. Differential PFPs in samples were screened out by two-dimensional fluorescence difference gel electrophoresis, and identified with matrix-assisted laser desorption/ionization-time of flight mass spectrometry; then the identified proteins were further authenticated by Western-blotting.</p><p><b>RESULTS</b>Thirteen differential PFPs were screened out, and among them the 7 identified by spectrometry were isoform 1 of integrin alpha- II b, isoform 2 of integrin alpha- II b, actin-cytoplasmic 1, actin-cytoplasmic 2, cDNA FLJ52842, cDNA FLJ55253, and cDNA FLJ43573 fis. Among them isoform 2 of integrin alpha- II b (CD41) and actin-cytoplasmic 2 (Acting) were authenticated successfully.</p><p><b>CONCLUSION</b>CD41 and acting are the possible marker proteins, and the other PFPs might play crucial roles in the occurrence and development of BSS in CHD.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Sangre
/
Plaquetas
/
Glicoproteínas de Membrana Plaquetaria
/
Electroforesis en Gel Bidimensional
/
Estudios de Casos y Controles
/
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
/
Enfermedad Coronaria
/
Diagnóstico
/
Medicina Tradicional China
Tipo de estudio:
Estudio diagnóstico
/
Estudio observacional
/
Estudio de tamizaje
Límite:
Adulto
/
Anciano
/
Femenino
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Año:
2010
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS