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Screening of the Presence of Enterovirus and Cytomegalovirus Infections in Terminally Failing Human Hearts
Korean Circulation Journal ; : 1527-1537, 1998.
Artículo en Coreano | WPRIM | ID: wpr-23153
ABSTRACT

BACKGROUND:

In order to evaluate the prevalence of enterovirus and cytomegalovirus infections to terminally failing hearts, the presence of enteroviral RNA and cytomegaloviral DNA was screened in the explanted hearts of transplantation recipients.

METHODS:

RNA and DNA extractions were performed from explanted failing hearts (N=22) and normal hearts (N=5). Reverse transcription-polymerase chain reaction (RT-PCR) of enterovirus and polymerase chain reaction (PCR) of cytomegalovirus were performed. In situ RT-PCR and in situ PCR were performed with positive nucleic acids of viruses.

RESULTS:

The positivity of enterovirus in failing hearts was 4.4% (1/22) and 0% (0/5) in normal hearts in nested RT-PCR. There was no significant difference in positivity of enteroviral RNA between failing and normal hearts. Nuclei of myocardium was stained in dark-violet color with in situ RT-PCR. The positivity of cytomegalovirus in failing hearts was 45% (10/22) and 40% (2/5) in nested PCR. There was no significant difference in positivity of cytomegaloviral DNA between failing and normal hearts. Nuclei of myocardium was stained in dark-violet color with in situ PCR. Positive chambers of cytomegalovirus were in decreasing tendency according to increasing patient's age.

CONCLUSION:

Enterovirus was very rarely observed in explanted terminally failing hearts and cytomegalovirus was frequently found both in explanted failing hearts and normal. These viruses have little direct causal relationship with the development of heart failure.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: ADN / ARN / Ácidos Nucleicos / Cardiomiopatía Dilatada / Tamizaje Masivo / Reacción en Cadena de la Polimerasa / Prevalencia / Enterovirus / Infecciones por Citomegalovirus / Citomegalovirus Tipo de estudio: Estudio diagnóstico / Estudio de prevalencia / Estudio de tamizaje Límite: Humanos Idioma: Coreano Revista: Korean Circulation Journal Año: 1998 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: ADN / ARN / Ácidos Nucleicos / Cardiomiopatía Dilatada / Tamizaje Masivo / Reacción en Cadena de la Polimerasa / Prevalencia / Enterovirus / Infecciones por Citomegalovirus / Citomegalovirus Tipo de estudio: Estudio diagnóstico / Estudio de prevalencia / Estudio de tamizaje Límite: Humanos Idioma: Coreano Revista: Korean Circulation Journal Año: 1998 Tipo del documento: Artículo