Effect of xuefu zhuyu decoction in inducing angiogenesis gene regulation of endothelial cell line ECV304 / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 153-156, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-231568
ABSTRACT
<p><b>OBJECTIVE</b>To study the acting mechanism of endothelial cell line ECV304 in regulating angiogenesis induced by Xuefu Zhuyu Decoction (XFZY).</p><p><b>METHODS</b>The angiogenesis effect of XFZY-contained serum (XFZY-CS) was confirmed by observing its impact on proliferation, cell cycle, migration of ECV304 and on vascular neogenesis in vitro. Then the effect of XFZY on various angiogenesis controlling factors was analyzed with gene chip microarray technique.</p><p><b>RESULTS</b>Treatment of XFZY-CS in 2.5% concentration for 48 h showed evident actions of enhancing ECV304 activity, increasing cell numbers of S phase, inducing cell migration and promoting the in vitro angiogenesis. Meanwhile, expressions of four angiogenesis controlling genes were up-regulated and 10 were down-regulated.</p><p><b>CONCLUSION</b>The angiogenesis mechanism of ECV304 induced by XFZY is complex, it shows a multi-pathway and multi-target feature.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Medicamentos Herbarios Chinos
/
Línea Celular
/
Regulación de la Expresión Génica
/
Ratas Sprague-Dawley
/
Neovascularización Fisiológica
/
Células Endoteliales
/
Inductores de la Angiogénesis
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Año:
2010
Tipo del documento:
Artículo
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