Regulation of Fc receptor expression by immune complexes on neutrophils and U937 cells / 中国医学科学院学报

ABSTRACT

<p><b>OBJECTIVE</b>To study the regulation of Fc receptor expression by immune complexes (ICs) on neutrophils and U937 cells.</p><p><b>METHODS</b>IgA ICs, IgG1 ICs, IgG2 ICs, IgG3 ICs, IgG4 ICs, and IgM ICs were incubated with neutrophils or U937 cells for 1 h. Then their surface Fc receptors were stained by anti-Fc gammaR I, anti-Fc gammaR II , anti-Fc gammaR III, and anti-Fc alphaR I monoclonal antibodies and analyzed by fluorescent activated cell sorting (FACS).</p><p><b>RESULTS</b>IgG1 ICs and IgG3 ICs up-regulated Fc gammaR II and Fc gammaR III on U937 cells, Fc gammaR I and Fc alphaR I on neutrophils. Almost all ICs down-regulated Fc gammaR II on neutrophils.</p><p><b>CONCLUSIONS</b>ICs can regulate Fc receptor expression on neutrophils and U937 cells, among which IgG1 ICs and IgG3 ICs are most effective.</p>