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Drug screening model acting on out-membrane protein OprM in pseudomonas aeruginosa efflux pump system / 中国医学科学院学报
Acta Academiae Medicinae Sinicae ; (6): 359-363, 2004.
Artículo en Chino | WPRIM | ID: wpr-231928
ABSTRACT
<p><b>OBJECTIVE</b>To establish an efflux pump inhibitor screening model with the out-membrane protein OprM in Pseudomonas aeruginosa efflux pump system as the target point.</p><p><b>METHODS</b>Efflux pump out-membrane protein gene oprM was obtained from standard Pseudomonas aeruginosa PA01 strain. Expression of OprM protein was induced in E. coli strain HS151 with T-easy vector as the cloning vector, and pMMB67EH as the expression vector. In order to evaluate the function of OprM protein, we measured intracellular tetracycline concentrations with liquid scintillation counter, measured the diameters of bacteriostatic circles with paper disc, and then established a screening model accordingly.</p><p><b>RESULTS</b>OprM protein was highly expressed. Using Pseudomonas aeruginosa as the main detecting bacteria, we established a drug screening model acting on OprM. A total of 1 600 microbial fermentation samples were screened with this model, among which 56 positive strains were found, with a positive rate of 3.5%.</p><p><b>CONCLUSION</b>OprM plays an important role in drug efflux. The established model has good specificity and maneuverability.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Proteínas de Transporte de Membrana / Farmacología / Plásmidos / Pseudomonas aeruginosa / Proteínas de la Membrana Bacteriana Externa / Proteínas Bacterianas / Farmacorresistencia Microbiana / Resistencia a Múltiples Medicamentos / Evaluación Preclínica de Medicamentos / Escherichia coli Tipo de estudio: Estudio diagnóstico / Estudio de tamizaje Límite: Humanos Idioma: Chino Revista: Acta Academiae Medicinae Sinicae Año: 2004 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Proteínas de Transporte de Membrana / Farmacología / Plásmidos / Pseudomonas aeruginosa / Proteínas de la Membrana Bacteriana Externa / Proteínas Bacterianas / Farmacorresistencia Microbiana / Resistencia a Múltiples Medicamentos / Evaluación Preclínica de Medicamentos / Escherichia coli Tipo de estudio: Estudio diagnóstico / Estudio de tamizaje Límite: Humanos Idioma: Chino Revista: Acta Academiae Medicinae Sinicae Año: 2004 Tipo del documento: Artículo