Dystrophin and utrophin expression in muscle tissues of DMD mouse model after transplantation treatment by bone marrow mesenchymal stem cells / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 294-297, 2004.
Artículo
en Chino
| WPRIM
| ID: wpr-231942
ABSTRACT
<p><b>OBJECTIVE</b>To observe dystrophin and utrophin expression in muscle tissues of Duchenne muscular dystrophy (DMD) mouse model (dko mouse) after having been treated with bone marrow mesenchymal stem cells (MSC) transplantation.</p><p><b>METHODS</b>The fifth generation of MSCs, cultured in vitro, was transplanted into dko mice by tail vein. The fluorescent expression of dystrophin and utrophin in gastrocnemius muscle tissue of dko mouse was detected and the average optical density of positive fibers was calculated.</p><p><b>RESULTS</b>MSCs that had been cultured for three generations had good homogeneousness and the immunological reaction after vein transplantation was low. There was an increasing tendency of dystrophin and utrophin fluorescent expression in sarcolemma of dko mouse within 5-20 weeks. Significant difference existed in fluorescent average optical density of positive fibers fifteen weeks before and after cell transplantation.</p><p><b>CONCLUSIONS</b>MSC has strong plasticity both in vitro and in vivo. MSC has a trend to reach the injured muscle tissues and turn into muscle fibers, which express dystrophin and utrophin. There is some plerosis function for myatrophy of dko mouse by MSC transplantation.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Cirugía General
/
Células de la Médula Ósea
/
Distrofina
/
Ratas Sprague-Dawley
/
Ratones Noqueados
/
Músculo Esquelético
/
Distrofia Muscular de Duchenne
/
Biología Celular
/
Proteínas del Citoesqueleto
/
Trasplante de Células Madre Mesenquimatosas
Límite:
Animales
Idioma:
Chino
Revista:
Acta Academiae Medicinae Sinicae
Año:
2004
Tipo del documento:
Artículo
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