Isolation and cloning of genes related to growth inhibition of human glioma BT325 by EGF / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 139-144, 2004.
Artículo
en Chino
| WPRIM
| ID: wpr-231972
ABSTRACT
<p><b>OBJECTIVE</b>To isolate and clone the differentially expressed genes induced by epithelial growth factor (EGF) with inhibiting dosage in cultured glioma BT325 cells and understand the molecular mechanism that inhibits glioma cells growth.</p><p><b>METHODS</b>Using differential display reversed transcription polymerase chain reaction (DDRT-PCR) method to analyze the differentially expressed cDNA in BT325 cells induced by EGF with inhibiting dosage. After sequencing and homology research, the differentially expressed cDNA fragments were further confirmed by Dot blot analysis and one of them by Northern blot.</p><p><b>RESULTS</b>Up-regulated genes cDNA fragments were isolated in growth inhibited BT325 cells. It was found that five cDNA fragments were highly homologous to the known human genes, while one was a fragment of a novel genes. Among these genes, one has coding sequence homology with transaldolase (TAL), which has been proved to be associated with apoptosis in recently research.</p><p><b>CONCLUSIONS</b>High-dose EGF could change the expression of many genes in BT325 cells. EGF can inhibit the growth of BT325 cell growth, which may be resulted from its potential role in promoting TAL gene expression and thus inducing cell apoptosis.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Neoplasias Encefálicas
/
Datos de Secuencia Molecular
/
Células Tumorales Cultivadas
/
Secuencia de Bases
/
Regulación Neoplásica de la Expresión Génica
/
División Celular
/
Genes Supresores de Tumor
/
Clonación Molecular
Límite:
Humanos
Idioma:
Chino
Revista:
Acta Academiae Medicinae Sinicae
Año:
2004
Tipo del documento:
Artículo
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