Enrichment of type A1-A4 spermatogonia by flow cytometry / 中华男科学杂志
National Journal of Andrology
;
(12): 3-6, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-231997
ABSTRACT
<p><b>OBJECTIVE</b>To explore a method to isolate and purify the subtype of type A spermatogonial stem cells (SSCs).</p><p><b>METHODS</b>We isolated spermatogonia by discontinuous density gradient centrifugation, sorted c-kit-expressed cells with the fluorescence-activated cell sorter (FACS), observed their ultrastructure by electron microscope, and performed immunohistochemistry to determine the expression of c-kit in the testis.</p><p><b>RESULTS</b>The c-kit positive cells constituted (18.65 +/- 1.69) % of the testis cells that were isolated by density gradient centrifugation, but made up only (3.16 +/- 0.84) % of those that were not (P < 0.01). The rates of recovery and viability of the c-kit positive cells sorted by FACS were (65.90 +/- 1.24)% and (85.60 +/- 1.14)%, respectively.</p><p><b>CONCLUSION</b>With c-kit as the marker, FACS can effectively isolate and purify the subtype of SSCs after preliminarily purified by discontinuous density gradient centrifugation.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Espermatogonias
/
Células Madre
/
Testículo
/
Microscopía Electrónica
/
Separación Celular
/
Ratas Sprague-Dawley
/
Proteínas Proto-Oncogénicas c-kit
/
Biología Celular
/
Citometría de Flujo
/
Metabolismo
Límite:
Animales
Idioma:
Chino
Revista:
National Journal of Andrology
Año:
2008
Tipo del documento:
Artículo
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