Inhibition of telomerase with hTERT antisense enhances TNF-alpha-induced apoptosis in prostate cancer cells PC3 / 中华男科学杂志
National Journal of Andrology
;
(12): 723-726, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-232076
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the inhibiting effect of telomerase with hTERT antisense on TNF-alpha-induced apoptosis in prostate cancer cells PC3.</p><p><b>METHODS</b>Antisense phosphorothioate oligodeoxynucleotide (AS PS-ODN) was synthesized and purified. Telomerase activity was measured by telomeric repeat amplification protocol (TRAP) and telomerase PCR-ELISA Kit, cell viability was determined by MTT assay, and cell apoptosis was observed by morphological method and determined by flow cytometry.</p><p><b>RESULTS</b>AS PS-ODN could significantly inhibit the telomerase activity and increase the susceptibility of TNF-alpha-induced apoptosis of PC3 cells.</p><p><b>CONCLUSION</b>Inhibition of telomerase with hTERT antisense can enhance TNF-alpha-induced apoptosis in prostate cancer cells.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Neoplasias de la Próstata
/
Ensayo de Inmunoadsorción Enzimática
/
Supervivencia Celular
/
Reacción en Cadena de la Polimerasa
/
Factor de Necrosis Tumoral alfa
/
Apoptosis
/
Telomerasa
/
Oligodesoxirribonucleótidos Antisentido
Límite:
Humanos
/
Masculino
Idioma:
Chino
Revista:
National Journal of Andrology
Año:
2007
Tipo del documento:
Artículo
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